Dr. Keith Nehrke

Dr. Keith Nehrke

CURRICULUM VITAE
KEITH WEBSTER NEHRKE Ph.D.


Work Address

Department of Medicine, Nephrology Division, Box 675
University of Rochester Medical Center
601 Elmwood Avenue, Rochester, NY 14642
Tel: (585) 275-7020 Fax: (585) 442-9201
Email: Keith_Nehrke@urmc.rochester.edu


View Home Address »

Home Address
1514 Rush-Scottsville Rd.
Rush, NY 14543
Tel: (585) 533-2294 Mobile: (585) 748-6652

View Personal Details »

Personal Details
Born: Morgantown, WV, August 19th, 1967
Married: Krystel Raluka Huxlin, Ph.D. Australian/American Citizen

View Higher Education »

Higher Education
Degree Year Institute / Discipline
B.S. (honors) 1989 Department of Biochemistry, State University of New York at Binghamton
Presidential Scholar (1985-1989)
Biochemistry, with honors.
M.S.
Ph.D.
1991
1994
Department of Biochemistry, University of Rochester
Dean’s Fellow
NIH Mechanisms of Molecular Pathogenesis Training Grant
Thesis: “mRNA 3’ end processing in bacteria and yeast.”
Mentor: Dr. Terry Platt

View Faculty Appointments »

Faculty Appointments
Year Institute / Discipline
1997 – 2002 Center for Oral Biology, University of Rochester Medical Center
Research Assistant Professor with Dr. James Melvin
2002 – 2003 Department of Medicine, Digestive Disease Division
Assistant Professor (Secondary Appointment, Pharmacology & Physiology)
2003 – 2010 Department of Medicine, Nephrology Division
Assistant Professor (Secondary Appointment, Pharmacology & Physiology)
2010 – 2015 Department of Medicine, Nephrology Division
Associate Professor (Secondary Appointment, Pharmacology & Physiology)
2015 – present Department of Medicine, Nephrology Division
Professor (Secondary Appointment, Pharmacology & Physiology)
Publications | A. Completed, Peer-Reviewed, Original Scientific Publications (mouse over the buttons for a synopsis of the work)

Generate list of PubMed citations containing Nehrke, K [author].

1. Phizicky E.M., Consaul S.A., Nehrke K.W., and Abelson J. (1992)
Yeast tRNA Ligase Mutants are Nonviable and Accumulate tRNA Splicing Intermediates. J Biol Chem. 267, 4577-4582.
2. Nehrke K., Seifried S.E., and Platt T. (1992)
Overproduced Rho Factor from p39AS has Lysine Replacing Glutamic Acid at Residue 155 in the Linker Region Between its RNA and ATP Binding Domains. Nucleic Acids Res. 20, 6107.
3. Nehrke K., Zalatan F., and Platt T. (1993)
NusG Alters Rho-Dependent Termination of Transcription In Vitro Independent of Kinetic Coupling. Gene Exp. 3, 119-133.
4. Nehrke, K. and Platt, T. (1994)
Evidence for the Formation of a Quaternary Termination Complex: Rho, NusG, RNA Polymerase and the Nascent Transcript. J Mol Biol. 243, 830-839
5. Russnak R., Nehrke K., and Platt T. (1995)
REF2 Encodes a Novel RNA-Binding Protein Directly Involved in Yeast mRNA 3′ End Formation. Mol Cell Biol. 15, 1689-1697
6. Nehrke K., Hagen F.H., and Tabak L.A. (1996)
Charge Distribution o Flanking Amino Acids Influences O-Glycan Aquisition in Vivo. J Biol Chem. 271, 7061-7065
7. Nehrke K. and Tabak L.A. (1997)
Biosynthesis of a Low Molecular Mass Rat Submandibular Gland Mucin in COS7 Cells. Biochem J. 323, 497-502
8. Nehrke K. Ten-Hagen K.G., Hagen F.K.,and Tabak L.A. (1998)
Charge Distribution of Flanking Amino Acids Inhibits O-Glycosylation of Several Single Site Acceptors In Vivo. Glycobiology 7,1053-1060
9. Nehrke K., Hagen F.K., and Tabak L.A. (1998)
Isoform-specific O-glycosylation by Murine UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3, in vivo. Glycobiology 8, 367-371
10. Hagen F.K. and Nehrke K. (1998)
cDNA Cloning and Expression of UDP-N-acetyl-D-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase sequence homologs in Caenorhabditis elegans J Biol Chem. 273, 8268-8277
11. Nehrke K., Begenisich T., Pilato J., and Melvin J.E. (2000)
C. elegans ClC-type Chloride Channels: Novel Variants and Functional Expression. Am J Physiol Cell Physiol., 279, C2052-C2066.
12. Melvin J.E., Nguyen H-V., Nehrke K., Schreiner C.M., Ten-Hagen K., and Scott W. (2001)
Targeted Disruption of the Nhe1 Gene Fails to Inhibit b1-Adrenergic Receptor-Induced Parotid Gland Hypertrophy. Am J Physiol Gastrointest Liver Physiol., 280, G694-G700.
13. Attaphitaya S., Nehrke K., and Melvin J.E. (2001)
Acute Inhibition of Brain-Specific Na+/H+ Exchanger Isoform 5 by Protein Kinase A, Protein Kinase C and Cell Shrinkage. Am J Physiol Cell Physiol., 281, C1146-C1157
14. Yan W., Nehrke K., Choi J. and Barber D. (2001)
The Nck-interacting kinase NIK phosphorylates the Na-H exchanger NHE1 and regulates NHE1 activation by platelet-derived growth factor. J Biol Chem., 276, 31349-31356
15. Park K., Evans R.L., Watson G., Nehrke K., Dennet M.R., Richardson L., Bell S., Schultheis P.J., Hand A., Shull G.E., and Melvin J.E. (2001)
Defective Fluid Secretion and NaCl Absorption by Parotid Glands in Mice Lacking Na-H Exchange. J Biol Chem., 276, 27042-27050.
16. Elble R., Gi J., Nehrke K., DiBiasio J., Kingsley P., Kotlikoff M.I., and Pauli B.U. (2002)
Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle. J Biol Chem., 277, 18586-18591
17. Nehrke K., Arreola J., Nguyen H-V., Pilato J., Richardson L., Okunade G., Shull G.E., Baggs R. and Melvin J.E. (2002)
Saliva Secretion and Cell Volume Regulation by Parotid Gland Acinar Cells from Mice Lacking Expression of the Clcn2 Cl- Channel Gene. J Biol Chem. 277:23604-23611
18. Nehrke K. and Melvin J.E. (2002)The NHX family of Na-H exchangers in Caenorhabditis elegans. J Biol Chem. 277:29036-29044
19. Arreola J., Begenisich T., Nehrke K., Nguyen H-V., Park K., Richardson L., Yang B., Lamb F., Shutte B.C., and Melvin J.E. (2002)
Secretion and Cell Volume Regulation by Salivary Acinar Cells from Mice Lacking Expression of the Clcn3 Cl- Channel Gene. J Physiol. 545:207-216
20. Dickerson L.W., Schutte B.C., Yang B., Bonthius D.J., Barna T.J., Bailey M.C., Nehrke K., Williamson R.A. and Lamb F.S. (2002)
Altered GABAergic function accompanies hippocampal degeneration in mice lacking ClC-3 voltage-gated chloride channels. Brain Research 958:227-250.
21. Nehrke K., Quinne C.C., and Begenisich T. (2003)
Molecular identification of the Ca2+-activated K+ channels in parotid acinar cells. Am J Physiol Cell Physiol. 284:C535-C546.
22. Joiner W.J., Basavappa S., Vidyasagar S., Nehrke K., Krishnan S., Binder H.J., Boulpaep E.L., and Rajendran V.M. (2003)
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon. Am J Physiol Gastrointest Liver Physiol. 285:G185-G196
23. Hoffman J.F., Joiner W., Nehrke K., Potapova O., Foye K., and Wickrema A. (2003)
The hSK4 (KCNN4) isoform is the Ca2+-activated K+ channel (Gardos channel) in human red blood cells. Proc Natl Acad Sci U S A. 100:7366-7371
24. Nehrke K. (2003)
A reduction in intestinal cell pHi due to loss of the C. elegans Na+/H+ exchanger NHX-2 increases lifespan. J Biol Chem 278:44657-44666
25. Chen Y., Zhao Y.H., Kalaslavadi T.B., Hamati E., Nehrke K., Le A.D., Ann D.K.,and Wu R. (2004)
Genome-wide search and identification of a novel gel-forming mucin MUC19/Muc19 in glandular tissues. Am J Respir Cell Mol Biol 30:155-165
26. Denton J., Nehrke K., Rutledge E., Morrison R. and Strange K. (2004)
Alternative splicing of N- and C-termini of a C. elegans ClC channel alters gating and sensitivity to external Cl- and H+. J Physiol 555: 97-114.
27. Begenisich T., Nakamoto T., Ovitt C., Nehrke K., Brugnara C., Alper S.L., Melvin J.E. (2004)
Physiological roles of the intermediate conductance, Ca2+-activated K channel, Kcnn4. J Biol Chem. [Epub ahead of print].
28. Denton J., Nehrke K., Yin X., Morrison R., and Strange K. (2005)
GCK-3, a newly identified Ste20 kinase, binds to and regulates the activity of a cell cycle-dependent ClC anion channel. J Gen Physiol. 125: 113-25
29. Sherman T., Chernova M.N., Clark J.S., Jiang L., Alper S.L., and Nehrke K. (2005)
The abts and sulp Families of Anion Transporters from C. elegans. Am J Physiol Cell Physiol. 289: C341-351
30. de Santiago J.A., Nehrke K., and Arreola J. (2005)
Quantitative Analysis of the Voltage-Dependent Gating of Mouse Parotid CLC-2 Chloride Channel. J Gen Physiol. 126: 591-603
31. Denton J., Nehrke K., Yin X., Beld A.M., and Strange K. (2006)
Altered gating and regulation of a carboxy-terminal ClC channel mutant expressed in the Caenorhabditis elegans oocyte. Am J Physiol Cell Physiol. 290: C1109-1118
32. He L., Denton J., Nehrke K., and Strange K. (2006)
Carboxy terminus splice variation alters ClC channel gating and extracellular cysteine reactivity. Biophys J. 90: 3570-3581
33. Yan X., Xing J., Lorin-Nebel C., Estevez A. Y., Nehrke K., Lamitina T., and Strange K. (2006).
Function of a STIM1 homologue in C. elegans: evidence that store-operated Ca2+ entry is not essential for oscillatory Ca2+ signaling and ER Ca2+ homeostasis. J Gen Physiol. 128: 443-459
34. Nehrke K., Denton J., and Mowrey W. (2008)
Intestinal Ca2+ wave dynamics in freely moving C. elegans coordinate execution of a rhythmic motor program. Am J Physiol Cell Physiol. 294: C333-344
35. Pfeiffer J., Johnson D., and Nehrke K. (2008)
Oscillatory transepithelial H+ flux regulates a rhythmic behavior in C. elegans. Curr Biol. 18: 297-302
36. Wojtovich A.P., Burwell L.S., Sherman T.A., Nehrke K. and Brookes P.S. (2008)
The C. elegans mitochondrial K+(ATP) channel: a potential target for preconditioning. Biochem Biophys Res Commun. 376: 625-628
37. Frick K.K., Krieger N.S., Nehrke K., and Bushinsky D.A. (2009)
Metabolic acidosis increases intracellular calcium in bone cells through activation of the proton receptor OGR1. J Bone Miner Res. 24: 305-313
38. Allman E., Johnson D., and Nehrke K. (2009)
Loss of the apical V-ATPase a-subunit VHA-6 prevents acidification of the intestinal lumen during a rhythmic behavior in C. elegans. Am J Physiol Cell Physiol. 297:C1071-81.
39. Wojtovich A.P., Williams D.M., Karcz M.C., Lopes C.M.B, Gray D.A., Nehrke K., and Brookes P.S. (2010)
A novel mitochondrial KATP channel assay. Circ. Res., 106:1190-1196.
40. Johnson D. and Nehrke K. (2010)
Mitochondrial fragmentation leads to intracellular acidification in C. elegans and mammalian cells. Mol Biol Cell. 21:2191-201
41. Wojtovich A.P., Nehrke K., and Brookes, P.S. (2010)
The mitochondrial complex II and ATP-sensitive potassium channel interaction: quantitation of the channel in heart mitochondria. Acta Biochim Pol. 57:431-434
42. Wagner J., Allman E., Taylor A., Ulmschneider K., Kovanda T., Ulmschneider B. Nehrke K., and Peters M.A. (2011) A calcineurin homologous protein is required for sodium-proton exchange events in the C. elegans intestine. Am J Physiol Cell Physiol. 301:C1389-1403
43. Wojtovich A.P., Sherman T.A., Nadtochiy S.M., Urciuoli W.R., Brookes P.S., and Nehrke K. (2011) SLO-2 is cytoprotective and contributes to mitochondrial potassium transport. PLoS One. 6:e28287
44. Sherman T.A., Rongali S.C., Matthews T.A., Pfeiffer J., and Nehrke K. (2012) Identification of a nuclear carbonic anhydrase in Caenorhabditis elegans. Biochim Biophys Acta. 1823:808-817.
45. Wojtovich A.P., Distefano P., Sherman T., Brookes P.S., and Nehrke K. (2012) Mitochondrial ATP-sensitive potassium channel activity and hypoxic preconditioning are independent of an inwardly rectifying potassium channel subunit in Caenorhabditis elegans. FEBS Lett. 586:428-34.
46. Johnson D., Allman E., and Nehrke K. (2012) Regulation of acid-base transporters by reactive oxygen species following mitochondrial fragmentation. Am J Physiol Cell Physiol.302 C1045-1054
47. Perry G.M., Nehrke K.W., Bushinsky D.A., Reid R., Lewandowski K.L., Hueber P., and Scheinman S.J. (2012) Sex modifies genetic effects on residual variance in urinary calcium excretion in rat (Rattus norvegicus). Genetics. 191:1003-1013
48. Portal-Celhay C., Nehrke K., and Blaser M.J. (2012) Effect of Caenorhabditis elegans age and genotype on horizontal gene transfer in intestinal bacteria. FASEB J. 27:760-768
49. Kemp B.J., Allman E., Immerman L., Mohnen M., Peters M.A., Nehrke K., and Abbott A.L. (2012) miR-786 regulation of a fatty-acid elongase contributes to rhythmic calcium-wave initiation in C. elegans. Curr Biol. 22:2213-2220
50. Queliconi B.B., Marazzi T.B., Vaz S.M., Brookes P.S., Nehrke K., Augusto O., and Kowaltowski A.J. (2012) Bicarbonate modulates oxidative and functional damage in ischemia-reperfusion. Free Radic Biol Med. 55:46-53
51. Wojtovich A.P., Nadtochiy S.M., Urciuoli W.R., Smith C.O., Grunnet M., Nehrke K., and Brookes P.S. (2013) A non-cardiomyocyte autonomous mechanism of cardioprotection involving the SLO1 BK channel. PeerJ. 1:e48
52. Allman E., Waters K., Ackroyd S., and Nehrke K. (2013) Analysis of Ca2+ signaling motifs that regulate proton signaling through the Na+/H+ exchanger NHX-7 during a rhythmic behavior in Caenorhabditis elegans. J Biol Chem. 288:5886-5895
53. Wojtovich A.P., Urciuoli W.R., Chatterjee S., Fisher A.B., Nehrke K., and Brookes P.S. (2013) Kir6.2 is not the mitochondrial KATP channel but is required for cardioprotection by ischemic preconditioning. Am J Physiol Heart Circ Physiol. 304:H1439-1445
54. Coburn C., Allman E., Mahanti P., Benedetto A., Cabreiro F., Pincus Z., Matthijssens F., Araiz C., Mandel A., Vlachos M., Edwards S.A., Fischer G., Davidson A., Pryor R.E., Stevens A., Slack F.J., Tavernarakis N., Braeckman B.P., Schroeder F.C., Nehrke K., and Gems D. (2013) Anthranilate fluorescence marks a calcium-propagated necrotic wave that promotes organismal death in Caenorhabditis elegans. PLoS Biol. 11: e1001613
55. Matthews T.A., Abel A., Demme C., Sherman T., Pan P.W., Halterman M.W., Parkkila S., and Nehrke K. (2014) Expression of the CHOP-inducible carbonic anhydrase CAVI-b is required for BDNF-mediated protection from hypoxia. Brain Res. 1543:28-37
56. Queliconi B.B., Kowaltowski A.J., and Nehrke K. (2014) An anoxia-starvation model for ischemia/reperfusion in C. elegans. J Vis Exp. (85)
57. Panitz D., Swamy H., and Nehrke K. (2015) A C. elegans model of electronic cigarette use: Physiological effects of e-liquids in nematodes. BMC Pharmacol Toxicol. 16:32
58. Allman E., Wang Q., Walker R.L., Austen M., Peters M.A., and Nehrke K. (2016) Calcineurin homologous proteins regulate the membrane localization and activity of sodium/proton exchangers in C. elegans. Am J Physiol Cell Physiol. 310(3):C233-42
59. Wojtovich A.P., Smith C.O., Urciuoli W.R., Wang Y.T., Xia X.M., Brookes P.S., and Nehrke K. (2016) Cardiac Slo2.1 Is Required for Volatile Anesthetic Stimulation of K+ Transport and Anesthetic Preconditioning. Anesthesiology 124(5):1065-76
60. Wojtovich A.P., Wei A.Y., Sherman T.A., Foster T.H., and Nehrke K. (2016) Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans. Scientific Reports 6:29695
61. Peña S., Sherman T.A., Brookes P.S., and Nehrke K. (2016) The Mitochondrial Unfolded Protein
Response Protects against Anoxia in Caenorhabditis elegans. PLoS One 11: e0159989
62. Nadtochiy S.M., Schafer X., Fu D., Nehrke K., Munger J and Brookes P.S. (2016) Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling. J Biol Chem. 291:20188-97
Publications | B. Invited Reviews
1. Hagen F., Layden M., Nehrke K., Gentile K., Berbach K., Tsao C.C., and Forsythe, M. (2001)
Mucin Type O-Glycosylation in C. elegans is Initiated by a Family of Glycosyltransferases. Trends in Glycoscience and Glycotechnology, 13: 463-479
2. Strange K., Denton J., and Nehrke K. (2006)
Ste20-type kinases: evolutionarily conserved regulators of ion transport and cell volume. Physiology. 21: 61-68.
3. Bushinsky D.A., Frick K.K., and Nehrke K. (2006)
Genetic hypercalciuric stone-forming rats. Curr Opin Nephrol Hypertens. 15: 403-418
4. Wojtovich A.P., Nadtochiy S.M., Brookes P.S., and Nehrke K. (2011)
Ischemic preconditioning: the role of mitochondria and aging. Exp Gerontol. 47:1-7.
5. Wojtovich A.P., Smith C.O., Haynes C.M., Nehrke K.W., Brookes P.S. (2013) Physiological consequences of complex II inhibition for aging, disease, and the mKATP channel. Biochim Biophys Acta. 1827:598-611. .
6. Nehrke K. (2016) H(OH), H(OH), H(OH): a holiday perspective. Focus on “Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH”. Am J Physiol Cell Physiol. 311:C942-C944
7. Smith C.O., Nehrke K., and Brookes P.S. (2017) The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection. Biochem J. 474:2067-2094
Publications | C. Book Chapters
1. Nehrke K., Hagen F.H., and Tabak L.A. (1997)
Experimental Approaches to Studying O-Glycosylation In Vivo. In: Techniques in Glycobiology, Ed. R. Townsend and A. Hotchkiss. Marcel Dekker. New York, NY.
2. Melvin J.E., Arreola J., Nehrke K., and Begenisich T. (2002)
Ca2+-Activated Cl- Currents in Salivary and Lacrimal Glands. In: Current Topics in Membranes. Vol. 53. Ed. C.M. Fuller. Academic Press. San Diego, CA.
3. Nehrke K. (2006)
Intracellular pH measurements in vivo using green fluorescent protein variants. In: C. elegans Methods and Applications. Ed. K. Strange. Methods Mol Biol. Vol. 351: 223-39
4. Allman E., Thyagarajan B., and Nehrke K. (2012)
The inositol 1,4,5-trisphospate receptor in C. elegans. In: Wiley Interdisciplinary Reviews: Membrane Transport and Signaling. Ed. M. Nedergaard and A. Verkhratsky. 1: 321–328.
5. Nehrke K. (2014)
Membrane Ion Transport in Non-excitable Tissues In: Wormbook. Ed. The C. elegans Research Community. doi: 10.1895/wormbook.1.174.1